Total cholesterol, triglycerides, and high-density lipoprotein (HDL-C) cholesterol were determined by enzymatic methods using commercial kits (Boehringer Mannheim, Germany). Plasma glucose concentration was determined twice by a glucose-oxidase method adapted to an Autoanalyzer (Hitachi 704, Boehringer Mannheim, Germany). Subjects were considered obese if their BMI was ≥ 30 kg/m 2.Īfter an overnight period, 20 ml of blood were obtained from an antecubital vein without compression. Individuals with a history of hyperlipidemia, hypertension, or diabetes mellitus were deemed to have their respective risk factors, regardless of the biochemical values. BP control was defined as < 140/90 mm Hg in nondiabetic subjects and < 140/85 mm Hg in type 2 diabetic subjects. Hypertension was diagnosed in those subjects treated with blood pressure medication and/or had a mean systolic BP ≥ 140 mm Hg or alternatively equal or higher of diastolic BP ≥ 90 mm Hg, according to the guidelines of the European Hypertension Society. Regarding BP control, it was defined as under control if BP was 1 year ago at the time of the study). Hypertension was diagnosed in pharmacology treated subjects or those with blood pressure (BP) ≥140/90 mm Hg. Medical questionnaires were administered and anthropometrics were measured, using standardized protocols. The prevalence and related factors of hypertensive subjects according to the resident area (rural versus urban) were investigated in two population-based studies from Spain.
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